So first in Opening Fcn add this two lines both in gui1 and gui2 Now create a blank GUI with a pushbutton titled "Start" and save it as gui0 Now in this file, go to gui Opening Fcn and you will find the followin code there handles.output = h Object; handles.s1= gui1; handles.s2= gui2; h1=guidata(handles.s1); h1= handles.s2; h1= h Object; guidata(handles.s1,h1); h2=guidata(handles.s2); h2= handles.s1; h2= h Object; guidata(handles.s2,h2); As you can see, I have called gui1 and gui2 and saved there reference or pointers to handles.s1 and handles.s2.
Now once you are done , you return to master page again because next slide of the s2 is masterpage.
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Now for navigation, we added the s2 to the next of s1(slide 1) and mastergui as previous of s1.